ABSTRACT
Objective To observe the change of peripheral blood Th17 cells and relationship between the severity and Th17 cells in patients with acute asthma.Methods We recruited patients with mild acute asthma(n=10) and severe acute asthma(n=10), and healthy volunteers(n=10). T-lymphocytes were collected from the peripheral blood mononuclear cells (PBMC). Flow cytometer (FCM) was used to detect the expression of peripheral blood Th17 cells. IL-17 levels in the peripheral blood were measured by enzyme-linked immunosorbentassay (ELISA).Results The rate of positive Th17 cells of peripheral blood in the severe acute asthma group was higher than that in the mild acute asthma group(P＜0.05) and the rate of positive Th17 cells of peripheral blood in healthy volunteer group were the lowest among all groups (P＜0.05, respectively). The level of IL-17 in the peripheral blood of patients with severe acute asthma increased significantly compared with that in patients with mild acute asthma and healthy volunteers (P＜0.05). The positive Th17 cells of peripheral blood in patients with acute asthma were positively correlated with the severity of acute asthma(r=0.869, P＜0.05).Conclusion The positive rate of Th17 cells in the peripheral blood increases in patients with acute asthma and has positive correlation with the severity of acute asthma.
ABSTRACT
Objective To study the change of live circulation in advanced chronic cor pulmonale.Methods Determination of blood gas,liver function and rheohepatogram were performed in twenty-eight patients with chronic cor pulmonale in acute attack period before treatment,and accounted for the cardiac output(CO),hepatic blood flow(HBF),hepatic artery blood flow(HABF) and portal vein blood flow(PVBF).Results The experimental group was compared with the control group,the HBF,HABF and PVBF were decreased,which was more dramatic in the patients with chronic cor pulmonale,who had right ventricular failure complication in the acute attack period.Conclusion In acute attack period of chronic cor pulmonale,the liver circulation obstruction occurs early,and it is the main cause of liver damage for the patients.